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Construction of full-length infectious cDNA clones of two Korean isolates of turnip mosaic virus breaking resistance in Brassica napus
Ist Teil von
Archives of virology, 2022-04, Vol.167 (4), p.1157-1162
Ort / Verlag
Vienna: Springer Vienna
Erscheinungsjahr
2022
Quelle
SpringerLink
Beschreibungen/Notizen
In this work, two new turnip mosaic virus (TuMV) strains (Canola-12 and Canola-14) overcoming resistance in canola (
Brassica napus
) were isolated from a
B. napus
sample that showed typical TuMV-like symptoms and was collected in the city of Gimcheon, South Korea, in 2020. The complete genome sequence was determined and an infectious clone was made for each isolate. Phylogenetic analysis indicated that the strains isolated from canola belonged to the World-B group. Both infectious clones, which used 35S and T7 promoters to drive expression, induced systemic symptoms in
Nicotiana benthamiana
and
B. napus.
To our knowledge, this is the first report of TuMV infecting
B. napus
in South Korea.