Details

Autor(en) / Beteiligte

• Lee, Jungwhoi
• Jo, Sung-Eun
• Lee, Jungsul
• Kim, Jae-Hoon

Titel

An in vitro evaluation of luffa cylindrica stem sap in preadipocytes and dermal fibroblasts

Ist Teil von

• Biochemical and biophysical research communications, 2022-04, Vol.599, p.100-105

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Luffa cylindrica stem sap (LuCS) has been ethnopharmacologically used as a cosmetic ingredients to improve the facial condition in Asians, but there is no scientific proof about the advantages of LuCS as a supplement for skin elasticity inducer. Presently, we have validated the beneficial effect of LuCS in human preadipocyte and fibroblast. In vitro activities of LuCS on expression of cellular elastin and collagen type I were validated using Western blot analysis in human fibroblasts. Effect of LuCS on preadipocyte development was performed using MDI medium containing isobutyl-methylxanthine, dexamethasone, and insulin and then evaluated using oil red O staining. Treatment of LuCS stimulated the expression of cellular elastin and type I procollagen in human skin fibroblasts. Exposure to LuCS induced lipid accumulation of preadipocytes via activation of CEBP/α signaling pathway in preadipocytes. Expression of collagen I, elastin, or CEBP/α mRNA was decreased by age. 3-bromo-3-methylisoxazol-5-amine enhanced the synthesis of cellular lipid in preadipocytes. Collectively, these results suggest the rationale of LuCS treatment in enhancing the skin condition. ●L. cylindrica stem sap regulates the expression levels of cellular type I collagen and elastin in human skin fibroblasts.●L. cylindrica stem sap induces the accumulation of lipid in human preadipocytes.●4-bromo-3-methylisoxazol-5-amine stimulates the lipid synthesis in human preadipocytes.●This is the report of the clearly effective 4-bromo-3-methylisoxazol-5-amine containing LuCS as an effective lipid inducer.