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A colorimetric sensor for Staphylococcus aureus detection based on controlled click chemical-induced aggregation of gold nanoparticles and immunomagnetic separation
Staphylococcus aureus
(
S. aureus
) is a pathogen closely associated with foodborne diseases. We prepared a reliable colorimetric sensor to detect
S. aureus
using click chemical reaction and immunomagnetic separation. Aptamer-functionalized and ALP-labeled Fe
3
O
4
NPs act as separation and signal transduction elements. Under the optimized conditions, the Cu
+
generated by signal transduction triggers a click chemistry reaction, which causes the aggregation of azides and alkyne-AuNPs and a color change. The net extinction ratio of Δ(A
530
/
A
760
) was linearly correlated with the
S. aureus
concentration from 10 to 10
6
cfu mL
−1
, and the limit detection was 2.4 cfu mL
−1
. The recoveries were 91.15 ~ 106.36% for the analysis of spiked food and water samples without pre-enrichment. Therefore, we believe that the detection platform can be easily and accurately used for
S. aureus
detection, providing a broad prospect for on-site visual detection.
Graphical abstract