Details

Autor(en) / Beteiligte

• Atapattu, Sanka N.

Titel

Retention properties of acetone‐water mobile phases on a biphenylsiloxane‐bonded silica stationary phase in reversed‐phase liquid chromatography

Ist Teil von

• Journal of separation science, 2022-05, Vol.45 (9), p.1487-1492

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2022

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• The solvation parameter model system constants and retention factors were used to interpret retention properties of 39 calibration compounds on a biphenylsiloxane‐bonded stationary phase (Kinetex biphenyl) for acetone‐water binary mobile phase systems containing 30–70% v/v. Variation in system constants, phase ratios, and retention factors of acetone‐water binary mobile phases systems were compared with more commonly used acetonitrile and methanol mobile phase systems. Retention properties of acetone mobile phases on a Kinetex biphenyl column were more similar to that of acetonitrile than methanol mobile phases except with respect to selectivity equivalency. Importantly, selectivity differences arising between acetone and acetonitrile systems (the lower hydrogen‐bond basicity of acetone‐water mobile phases and differences in hydrogen‐bond acidity, cavity formation and dispersion interactions) could be exploited in reversed‐phase liquid chromatography method development on a Kinetex biphenyl stationary phase.