Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Genetic variation analysis of the sigma B protein gene of novel duck reovirus in southeastern China from 2011 to 2020
Ist Teil von
Journal of virological methods, 2022-05, Vol.303, p.114479-114479, Article 114479
Ort / Verlag
Netherlands: Elsevier B.V
Erscheinungsjahr
2022
Quelle
ScienceDirect Pay Per View(PPV) Titles
Beschreibungen/Notizen
•Reflecting the genetic variation of the sigma protein gene of the fourteen Novel duck reovirus in southeastern China.•Comparison of the amino acid properties and hydrophilicity of the sigma B protein gene encoded in different strains.•These findings demonstrate the need for and effectiveness of preventive measures targeting sigma B protein gene.
The novel duck reovirus (NDRV) disease first appeared in China in 2011. Infected ducks may be of various ages and breeds. Sigma B protein, which is the key component of NDRV's outer capsid, may trigger group-specific neutralizing antibodies linked to NDRV infection, pathogenicity, and immune defense. The sigma B protein gene of fourteen NDRV field strains was amplified by RT-PCR and cloned into the pMD-18 T vector for sequencing to examine the genetic variation in sigma B proteins of NDRVs in southeastern China between 2011 and 2020. The sigma B protein gene of the fourteen NDRV southeastern strains included in this analysis had 96.3 %–99.8 % nucleotide, and 96.2 %–99.7 % deduced amino acid sequence homology. Phylogenetic analysis revealed that the fourteen southeastern strains belonged to a well-supported lineage that included NDRV and Muscovy duck reovirus (MDRV) strains. However, Avian reovirus (ARV) formed a distinct genetic lineage in the gene tree. The sigma B protein gene sequences of NDRV strains found in southeastern China are substantially conserved, according to these findings. There is no significant geographical difference between NDRV southeastern strains and DRV strains in other regions of China. Our findings will add to the molecular epidemiological picture of NDRV strain spread in southeastern China between 2011 and 2020, laying the groundwork for potential in-depth research on vaccine collection and comprehensive prevention.