Details

Autor(en) / Beteiligte

• Navazas, Alejandro
• Mesa, Victoria
• Thijs, Sofie
• Fuente-Maqueda, Francisco
• Vangronsveld, Jaco
• Peláez, Ana I.
• Cuypers, Ann
• González, Aida

Titel

Bacterial inoculant-assisted phytoremediation affects trace element uptake and metabolite content in Salix atrocinerea

Ist Teil von

• The Science of the total environment, 2022-05, Vol.820, p.153088-153088, Article 153088

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2022

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Natural plant-associated microorganisms are of critical importance to plant growth and survival in field conditions under toxic concentrations of trace elements (TE) and these plant-microbial processes can be harnessed to enhance phytoremediation. The total bacterial diversity from grey willow (Salix atrocinerea) on a brownfield heavily-polluted with lead (Pb) and arsenic (As) was studied through pyrosequencing. Culturable bacteria were isolated and in vitro tested for plant growth-promotion (PGP) traits, arsenic (As) tolerance and impact on As speciation. Two of the most promising bacterial strains - the root endophyte Pantoea sp. AV62 and the rhizospheric strain Rhodococcus erythropolis AV96 - were inoculated in field to S. atrocinerea. This bioaugmentation resulted in higher As and Pb concentrations in both, roots and leaves of bacterial-inoculated plants as compared to non-inoculated plants. In consequence, bacterial bioaugmentation also affected parameters related to plant growth, oxidative stress, the levels of phytochelatins and phenylpropanoids, together with the differential expression of genes related to these tolerance mechanisms to TE in leaves. This study extends our understanding about plant-bacterial interactions and provides a solid basis for further bioaugmentation studies aiming to improve TE phytoremediation efficiency and predictability in the field. [Display omitted] •Salix atrocinerea's root-associated bacteria growing on a brownfield is analyzed.•Soil, rizo- and endosphere are rich in Pantoea, Pseudomonas and Rhodococcus, resp.•Selected bacterial inoculants present As-resistance and plant growth-promotion traits.•Salix inoculation with Pantoea sp. and R. erythropolis increases As and Pb removal.•Inoculation alters the levels of non-protein thiols and phenylpropanoid compounds.