Details

Autor(en) / Beteiligte

• Takeuchi, Chihiro
• Sato, Junichi
• Yamashita, Satoshi
• Sasaki, Akiko
• Akahane, Takemi
• Aoki, Rika
• Yamamichi, Mitsue
• Liu, Yu-Yu
• Ito, Masayoshi
• Furuta, Takahisa
• Nakajima, Shigemi
• Sakaguchi, Yoshiki
• Takahashi, Yu
• Tsuji, Yosuke
• Niimi, Keiko
• Tomida, Shuta
• Fujishiro, Mitsuhiro
• Yamamichi, Nobutake
• Ushijima, Toshikazu

Titel

Autoimmune gastritis induces aberrant DNA methylation reflecting its carcinogenic potential

Ist Teil von

• Journal of gastroenterology, 2022-03, Vol.57 (3), p.144-155

Ort / Verlag

Singapore: Springer Singapore

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• Background Autoimmune gastritis (AIG) is a chronic inflammatory condition in gastric mucosa and is associated with increased cancer risk, though not as high as that by Helicobacter pylori ( H. pylori )-associated gastritis (HPG). Although aberrant DNA methylation is induced by HPG and the level correlates with the risk of gastric cancer, DNA methylation induction by AIG is unknown. Methods Gastric mucosa samples from the corpus were collected from 12 people with AIG without H. pylori infection, 10 people with HPG, and eight healthy volunteers. Genome-wide DNA methylation analysis was conducted using Infinium Methylation EPIC array. Gene expression was analyzed by quantitative RT-PCR. Results The AIG samples had extensive aberrant DNA methylation but presented unique methylation profiles against the HPG samples after correction of leucocyte fractions. Comparison between the AIG and HPG samples showed that AIG induced methylation, but less than HPG, in overall CpG sites and also in promoter CpG islands. Promoter CpG islands of tumor-suppressor genes in the pathway of cell cycle, cell adhesion, p53, and WNT were highly methylated in the AIG samples, but more so in the HPG samples. The expression levels of IL1B and IL8 , secreted by macrophage, were significantly lower in the AIG samples than in the HPG samples, suggesting that a difference in inflammatory response affected the degree and patterns of aberrant DNA methylation. Conclusions AIG induced aberrant DNA methylation in gastric mucosa. However, the degree of DNA methylation was less than that by HPG, which reflected carcinogenic risk.