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Details

Autor(en) / Beteiligte
Titel
Driver mutations in ADGRL3 are involved in the evolution of ependymoma
Ist Teil von
  • Laboratory investigation, 2022-07, Vol.102 (7), p.702-710
Ort / Verlag
United States: Elsevier Inc
Erscheinungsjahr
2022
Quelle
MEDLINE
Beschreibungen/Notizen
  • Although there have been recent advances in the molecular pathology of ependymomas, little is known about the underlying molecular evolution during its development. Here, we assessed the clinical, pathological and molecular evolutionary process of ependymoma recurrence in a 9-year-old patient who had seven recurrences of supratentorial ependymoma and died from intracranial multiregional recurrences at the age of 19 years old. Whole-genome sequencing (WGS) of 7 tumor samples (1 primary and 6 subsequent recurrent tumors) was performed to elucidate the mutation landscape and identify potential driver mutations for tumor evolution. The genetic profiles of the seven tumor specimens showed significant heterogeneity and suggested a highly branched evolutionary pattern. The mutational signatures and chromothripsis changed with treatments. Strikingly, adhesion G protein-coupled receptor L3 (ADGRL3, also known as Latrophilins 3, LPNH3) was found to be consistently mutated during the entire disease process. However, Sanger sequencing of other 78 ependymoma patients who underwent surgery at our institution showed no genetic alteration of ADGRL3, as found in the present case. The mRNA levels of ADGRL3 were significantly lower in ependymomas (n = 36), as compared with normal brain tissue (n = 3). Grade III ependymomas had the lowest ADGRL3 expression. Moreover, ependymomas with lower mRNA level of ADGRL3 had shorter overall survival. Our findings, therefore, demonstrate a rare evolutionary process of ependymoma involving ADGRL3.
Sprache
Englisch
Identifikatoren
ISSN: 0023-6837
eISSN: 1530-0307
DOI: 10.1038/s41374-021-00721-3
Titel-ID: cdi_proquest_miscellaneous_2618905966

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