Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Complete genome sequence of a new orthotospovirus associated with ringspot in Fatsia japonica
Ist Teil von
Archives of virology, 2022-02, Vol.167 (2), p.615-618
Ort / Verlag
Vienna: Springer Vienna
Erscheinungsjahr
2022
Quelle
MEDLINE
Beschreibungen/Notizen
Fatsia japonica
is an evergreen shrub native to Japan. For decades, virus-like ringspot symptoms have been observed on leaves of
F. japonica
in Japan; however, previous attempts to identify the causal agents have been unsuccessful. In this study, we detected an orthotospovirus-like sequence in symptomatic
F. japonica
plants using RNA sequencing analysis. The complete nucleotide sequences of the L, M, and S segments of the virus were determined using conventional sequencing strategies. The virus had a typical orthotospovirus genome structure, and the putative nucleocapsid protein showed the highest sequence identity to that of groundnut chlorotic fan-spot virus, with 83.7% identity at the amino acid level (which is below the 90% species demarcation cutoff for the genus
Orthotospovirus
). Although we could not confirm the pathogenicity of the virus in
F. japonica
due to difficulties associated with mechanical inoculation, its association with the observed symptoms was suggested by the fact that the virus was detected only in symptomatic leaf areas. Based on these results, we consider this virus, which we have named "Fatsia japonica ringspot-associated virus" (FjRSaV), to be the first representative of a new orthotospovirus species, for which we propose the binomial "
Orthotospovirus fatsiae
".