Details

Autor(en) / Beteiligte

• Cioates Negut, Catalina
• Stefan-van Staden, Raluca-Ioana
• Badulescu, Marius
• Bita, Bogdan

Titel

Disposable stochastic sensors obtained by nanolayer deposition of copper, graphene, and copper-graphene composite on silk for the determination of isocitrate dehydrogenases 1 and 2

Ist Teil von

• Analytical and bioanalytical chemistry, 2022-02, Vol.414 (5), p.1797-1807

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2022

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Three disposable stochastic sensors designed using nanolayer deposition of copper (Cu), graphene (GR), and copper-graphene (Cu-GR) composite on the silk textile, as substrate, were modified with chitosan ( n =371–744), for biomedical analysis. Isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2) served as model analytes for molecular recognition and quantification in biological samples such as whole blood and brain tumor tissue samples. The best sensitivities (3.77×10 7 s μg mL −1 for IDH1, and 1.88×10 7 s μg mL −1 for IDH2) and the lowest limits of quantification (10 −2 fg mL −1 for IDH1, and 5×10 −2 fg mL −1 for IDH2) for both IDH1 and IDH2 were recorded for the disposable stochastic sensors based on chitosan/graphene nanolayer. Very good correlations between the screening method based on disposable stochastic sensors and enzyme-linked immunosorbent assay (ELISA) were obtained; this was also proved by the results obtained using the paired t -test. Graphical abstract