Details

Autor(en) / Beteiligte

• Schnell, Anna
• Davrandi, Mehmet
• Saxenhofer, Moritz
• Leboreiro, Clara
• Graeter, Stefanie
• Moreira, Fernando
• Hauswald, Maria
• Witte, Carolin
• Irincheeva, Irina
• Feussner, Annette
• Vonarburg, Cedric
• Schulze, Ilka
• Schaub, Alexander
• Burns, Siobhan O.
• Lowe, David M.

Titel

Airway inflammation and dysbiosis in antibody deficiency despite the presence of IgG

Ist Teil von

• Journal of allergy and clinical immunology, 2022-06, Vol.149 (6), p.2105-2115.e10

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Patients with antibody deficiency suffer chronic respiratory symptoms, recurrent exacerbations, and progressive airways disease despite systemic replacement of IgG. Little is known about the respiratory tract biology of these patients. We sought to measure immunoglobulin levels, inflammatory cytokines, and mediators of tissue damage in serum and sputum from patients with antibody deficiency and healthy controls; to analyze the respiratory microbiome in the same cohorts. We obtained paired sputum and serum samples from 31 immunocompetent subjects and 67 antibody-deficient patients, the latter divided on computed tomography scan appearance into “abnormal airways” (bronchiectasis or airway thickening) or “normal airways.” We measured inflammatory cytokines, immunoglobulin levels, neutrophil elastase, matrix-metalloproteinase-9, urea, albumin, and total protein levels using standard assays. We used V3-V4 region 16S sequencing for microbiome analysis. Immunodeficient patients had markedly reduced IgA in sputum but higher concentrations of IgG compared with healthy controls. Inflammatory cytokines and tissue damage markers were higher in immunodeficient patients, who also exhibited dysbiosis with overrepresentation of pathogenic taxa and significantly reduced alpha diversity compared with immunocompetent individuals. These differences were seen regardless of airway morphology. Sputum matrix-metalloproteinase-9 and elastase correlated inversely with alpha diversity in the antibody-deficient group, as did sputum IgG, which correlated positively with several inflammatory markers, even after correction for albumin levels. Patients with antibody deficiency, even with normal lung imaging, exhibit inflammation and dysbiosis in their airways despite higher levels of IgG compared with healthy controls.