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•An immunochromatographic test strip is designed for multiple mycotoxin detection.•It sensitively detects AFB1/OTA/ZEN with detection limits of 0.01/0.2/0.032 ng/mL.•It is proved with good selectivity tested by mixed mycotoxin solutions.•It is stable since it can be preserved nearly for one year at 4 °C.•It supports rapid multiple mycotoxin on-site detection within 45 min.
In this work, we design a sensitive and quantitative on-site detecting solution for Aflatoxin B1 (AFB1), Ochratoxin A (OTA) and Zearalenone (ZEN) as often found in moldy grains and harmful to human health. Using quantum dot microsphere-based immunochromatography test strip, the proposed method can sensitively detect AFB1, OTA and ZEN in low detection limits of 0.01 ng/mL, 0.2 ng/mL and 0.032 ng/mL, and quantitatively measure their concentrations from 0.01 ng/mL to 1 ng/mL, from 0.2 ng/mL to 200 ng/mL and from 0.032 ng/mL to 32 ng/mL in high accuracy and good selectivity. More importantly, these multiple mycotoxin detections only relying on simple manual operations and portable handheld test strip reader can be finished on site within 45 min. Therefore, the proposed method is a promising solution supporting sensitive and quantitative on-site detections for multiple mycotoxins.