Details

Autor(en) / Beteiligte

• Li, Weiyang
• Wei, Wei
• Hou, Fei
• Xu, Hanshi
• Cui, Xiaofang

Titel

The integration model of hepatitis B virus genome in hepatocellular carcinoma cells based on high-throughput long-read sequencing

Ist Teil von

• Genomics (San Diego, Calif.), 2022-01, Vol.114 (1), p.23-30

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• HBV integration and function has gradually been expanding. However, the exact mode of HBV integration remains unclear. In our research, the high-throughput long-read sequencing was combined with bioinformatics to study the complete mode of HBV integration in hepatocellular carcinoma (HCC) cells. The results demonstrated that: 1) The HBV insertion sequences of HBV integration events accounted for 49.5% of the total HBV sequences. 2) Short insertion segments with the length of 0–1 kbp accounted for 50% and the long insertion segments (>3 kbp) accounted for 25% of HBV insertion events. 3)There were different HBV insertion length in the breakpoints formed within different regions. 4) The occurrence of HBV integration events was accompanied by more frequent structural variations. 5)Furthermore, multiple HBV integration patterns were confirmed based on complete HBV insertion sequences. Our research not only clarified a variety of perfect HBV integration models but also determined multiple specific features of HBV integration. •The complete, diverse and complex HBV insertion sequences were discovered.•.•HBV integration events related to deletion length and the chromosome translocation event.•The insertion length of HBV genome is related to the region of breakpoints occurring.•The viral integration sequence can span the end of linear HBV genome.•HBV integration is a very important way of HBV existence in HCC cells.