Details

Autor(en) / Beteiligte

• Wang, Zhiqing
• Mu, Yao
• Hao, Xuefeng
• Yang, Jinbao
• Zhang, Daixuan
• Jin, Zhuping
• Pei, Yanxi

Titel

H2S aids osmotic stress resistance by S-sulfhydration of melatonin production-related enzymes in Arabidopsis thaliana

Ist Teil von

• Plant cell reports, 2022-02, Vol.41 (2), p.365-376

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Key message Hydrogen sulfide closed Arabidopsis thaliana stomata by increasing the transcription of melatonin-producing enzymes and the post-translational modification levels to combat osmotic stress . Hydrogen sulfide (H 2 S) and melatonin (MEL) reportedly have similar functions in many aspects of plant growth, development and stress response. They regulate stomatal movement and enhance drought resistance. However, their physiological relationship is not well understood. Here, their crosstalk involved in osmotic stress resistance in Arabidopsis thaliana was studied. Exogenous H 2 S and MEL closed stomata under normal or osmotic stress conditions and increased the relative water contents of plants under osmotic stress conditions. At the same time, exogenous H 2 S and MEL responded to osmotic stress by increasing the content of proline and soluble sugar, and reducing malondialdehyde (MDA) content and relative conductivity. Using mutants in the MEL-associated production of serotonin N -acetyltransferase ( snat ), caffeic acid O -methyltransferase ( comt1 ) and N -acetylserotonin methyltransferase ( asmt ), we determined that H 2 S was partially dependent on MEL to close stomata. Additionally, the overexpression of ASMT promoted stomatal closure. Exogenous H 2 S increased the transcription levels of SNAT, ASMT and COMT1. Furthermore, exogenous H 2 S treatments increased the endogenous MEL content significantly. At the post-translational level, H 2 S sulfhydrated the SNAT and ASMT, but not COMT1, enzymes associated with MEL production. Thus, H 2 S appeared to promote stomatal closure in response to osmotic stress by increasing the transcription levels of MEL synthesis-related genes and the sulfhydryl modification of the encoded enzymes. These results increased our understanding of H 2 S and MEL functions and interactions under osmotic stress conditions.