Details

Autor(en) / Beteiligte

• van Riemsdijk, Isolde
• Arntzen, Jan W.
• Babik, Wiesław
• Bogaerts, Sergé
• Franzen, Michael
• Kalaentzis, Konstantinos
• Litvinchuk, Spartak N.
• Olgun, Kurtuluş
• Wijnands, Jan Willem P.M.
• Wielstra, Ben

Titel

Next-generation phylogeography of the banded newts (Ommatotriton): A phylogenetic hypothesis for three ancient species with geographically restricted interspecific gene flow and deep intraspecific genetic structure

Ist Teil von

• Molecular phylogenetics and evolution, 2022-02, Vol.167, p.107361-107361, Article 107361

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• [Display omitted] •Next-generation phylogeography supports three banded newt species (Ommatotriton)•Reasonable support for one out of the three potential bifurcating phylogenies.•No extensive introgression (as is observed in other newts in the Near East)•Deep intraspecific genetic divergence only partially reflected by taxonomy.•Mitochondrial and nuclear DNA generally agree, but there is also clear discordance. Technological developments now make it possible to employ many markers for many individuals in a phylogeographic setting, even for taxa with large and complex genomes such as salamanders. The banded newt (genus Ommatotriton) from the Near East has been proposed to contain three species (O. nesterovi, O. ophryticus and O. vittatus) with unclear phylogenetic relationships, apparently limited interspecific gene flow and deep intraspecific geographic mtDNA structure. We use parallel tagged amplicon sequencing to obtain 177 nuclear DNA markers for 35 banded newts sampled throughout the range. We determine population structure (with Bayesian clustering and principal component analysis), interspecific gene flow (by determining the distribution of species-diagnostic alleles) and phylogenetic relationships (by maximum likelihood inference of concatenated sequence data and based on a summary-coalescent approach). We confirm that the three proposed species are genetically distinct. A sister relationship between O. nesterovi and O. ophryticus is suggested. We find evidence for introgression between O. nesterovi and O. ophryticus, but this is geographically limited. Intraspecific structuring is extensive, with the only recognized banded newt subspecies, O. vittatus cilicensis, representing the most distinct lineage below the species level. While mtDNA mostly mirrors the pattern observed in nuclear DNA, all banded newt species show mito-nuclear discordance as well.