Details

Autor(en) / Beteiligte

• Hou, Lele
• Qiu, Huiling
• Zhu, Lianqin
• Gao, Shansong
• Chen, Fu

Titel

Selenide Chitosan Sulfate Improved the Hepatocyte Activity, Growth Performance, and Anti-oxidation Capacity by Activating the Thioredoxin Reductase of Chickens In Vitro and In Vivo

Ist Teil von

• Biological trace element research, 2022-08, Vol.200 (8), p.3798-3807

Ort / Verlag

New York: Springer US

Erscheinungsjahr

2022

Quelle

SpringerNature Journals

Beschreibungen/Notizen

• Chicken hepatocytes were cultured in vitro and 240 specific pathogen-free (SPF) white leghorns chickens (7 days old) were obtained. The hepatocytes and chickens were randomly allocated to one of six treatment groups: control group; chitosan (COS) group; sodium selenite (Na 2 SeO 3 ) group; selenide chitosan (COS-Se) group; chitosan sulfate (LS-COS) group; and selenide chitosan sulfate (LS-COS-Se) group. Our results showed that LS-COS-Se increased ( P  < 0.05) the activities of thioredoxin reductase (TXNRD), anti-superoxide anion radical (antiO 2 − ), and superoxide dismutase (SOD), the mRNA levels of thioredoxin reductase 1 (TXNRD1) and thioredoxin reductase 3 (TXNRD3), and the chicken body weight, but reduced ( P  < 0.05) the malondialdehyde (MDA) content and the lactate dehydrogenase (LDH) activity. Compared with COS and LS-COS, the LS-COS-Se treatment increased ( P  < 0.05) the activities of TXNRD, SOD, catalase (CAT), and the mRNA levels of TXNRD1 and TXNRD3, but reduced ( P  < 0.05) the MDA content in vitro, whereas, in vivo, it increased ( P  < 0.05) body weight on day 28; the activities of TXNRD, antiO 2 − , and SOD; and the mRNA levels of TXNRD1 and TXNRD3. Compared with Na 2 SeO 3 and COS-Se, the LS-COS-Se treatment increased ( P  < 0.05) the TXNRD and SOD activities, the mRNA levels of TXNRD1 and TXNRD3 in vitro, increased ( P  < 0.05) the chicken body weight on day 28, and the TXNRD, antiO 2 − , and SOD activities, but reduced ( P  < 0.05) the MDA content. These results indicated that LS-COS-Se was a useful antioxidant that improved hepatocyte activity, growth performance, and anti-oxidation capacity in hepatocytes (in vitro) and SPF chicken (in vivo) by activating the TXNRD system.