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To differentiate
isolates from diarrheal pediatric patients in clinical laboratories.
Patients with watery diarrhea were selected for sampling and tested for diarrheagenic
(DEC) by API kit. DEC isolates were tested for phylotyping, pathotyping and presence of determined virulence-encoding genes by specific molecular methods.
About 50% of isolates were detected as DECs (>55 and >31% were categorized B2 and D phylotypes respectively). Enterotoxigenic
was the most and enteroinvasive
was the lowest prevalent pathotypes.
and
genes were the most present virulence factors.
Typing of
isolates from stool specimens will help to determine the diversity of diarrheal pathogens and take proper decisions to reduce the health burden of diarrheal diseases.