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Delayed body development with reduced triglycerides levels in leptin transgenic pigs
Ist Teil von
Transgenic research, 2022-02, Vol.31 (1), p.59-72
Ort / Verlag
Cham: Springer International Publishing
Erscheinungsjahr
2022
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
Leptin is a well-known adipokine that plays critical role in adiposity. To further investigate the role of leptin in adiposity, we utilized leptin overexpressing transgenic pigs and evaluated the effect of leptin on growth and development, fat deposition, and lipid metabolism at tissue and cell level. Leptin transgenic pigs were produced and divided into two groups: elevated leptin expression (leptin ( +)) and normal leptin expression group (control). Results indicated that leptin ( +) pigs had elevated leptin protein and mRNA expression levels and exhibited sluggish growth and development followed by decreased subcutaneous fat thickness, low serum triglycerides, saturated, unsaturated fatty acids and high cholesterol esters (
p
< 0.05). There were differences in the lipid metabolism related genes at different fat depots, including upregulation of
PPARγ
,
AGPAT6
,
PLIN2
,
HSL
and
ATGL
in subcutaneous,
PPARγ
in perirenal, and
FAT/CD36
and
PLIN2
in mesenteric adipose tissues and downregulation of
AGPAT6
and
ATGL
in perirenal and
AGPAT6
in mesenteric adipose tissues (
p
< 0.05). Additionally,
in-vitro
cultured leptin ( +) preadipocytes exhibited upregulation of
PPARγ, FAT/CD36, ACACA, AGPAT, PLIN2, ATGL
and
HSL
as compared to control (
p
< 0.05). These findings suggested that homeostasis imbalance in lipolysis and lipogenesis at adipose tissue and adipocytes levels led to low subcutaneous fat depots in leptin overexpression pigs. These pigs can act as model for obesity and related metabolic disorder.