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Involvement of the fatty acid-binding protein in the growth of Schistosoma japonicum schistosomula
Ist Teil von
Parasitology research (1987), 2021-11, Vol.120 (11), p.3851-3856
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2021
Link zum Volltext
Quelle
SpringerNature Journals
Beschreibungen/Notizen
This study aimed to explore the effect and mechanism underlying the role of the
Schistosoma japonicum
antigen of fatty acid-binding protein (
Sj
FABP) on the growth of the schistosomula.
SjFABP
levels were evaluated by quantitative real-time polymerase chain reaction of samples of mice infected with
S. japonicum
;
SjFABP
was expressed and its levels gradually increased during all stages of
S. japonicum
schistosomula, including on 3, 10, 14, and 21 days of the growth process. Immunohistochemistry results demonstrated that
Sj
FABP was distributed in the parenchyma, especially in the digestive tract of the
S. japonicum
schistosomula. RNA interference resulted in more than 60% knockdown of
Sj
FABP leading to a reduction in length, volume, width, and area of the schistosomula as compared to control samples, as determined by light microscopy. Terminal deoxynucleotidyl transferase dUTP nick-end labeling detection further suggested that
Sj
FABP knockdown resulted in increased apoptosis of schistosomes. Taken together, these results suggest that
Sj
FABP may be related to the growth and survival of
S. japonicum
schistosomula, thereby representing a potential target for the treatment of schistosomiasis.