Details

Autor(en) / Beteiligte

• Barhate, Chandan L
• Donnell, Andrew F
• Davies, Merrill
• Li, Ling
• Zhang, Yong
• Yang, Fukang
• Black, Regina
• Zipp, Greg
• Zhang, Yingru
• Cavallaro, Cullen L
• Priestley, E. Scott
• Weller, Harold N

Titel

Microscale purification in support of high-throughput medicinal chemistry

Ist Teil von

• Chemical communications (Cambridge, England), 2021-10, Vol.57 (84), p.1137-114

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• In recent years, successful assay miniaturization has enabled the exploration of synthesis scale reduction in pharmaceutical discovery. Miniaturization of pharmaceutical synthesis and purification allows a reduction in material consumption and shortens timelines, which ultimately reduces the cost per experiment without compromising data quality. Isolating and purifying the compounds of interest is a key step in the library synthesis process. In this manuscript we describe a high-throughput purification workflow in support of microscale (1-5 μmol or 0.5-2 mg) library synthesis. The optimized microscale purification system can routinely purify 384-well reaction plates with an analysis time of 4 min per sample. Instrument optimization, critical parameters such as column loading, delay time calibration, ultrafast pre- and post-purification analysis and library purification examples are provided. High-throughput purification workflow in support of microscale (1-5 μmol or 0.5-2 mg) library synthesis.