Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Polymorphisms of the prion‐related protein gene are strongly associated with cervids’ susceptibility to chronic wasting disease
Ist Teil von
Veterinary record, 2022-02, Vol.190 (4), p.no-no
Ort / Verlag
England: Blackwell Publishing Ltd
Erscheinungsjahr
2022
Quelle
Wiley-Blackwell Full Collection
Beschreibungen/Notizen
Background
Chronic wasting disease (CWD) is a cervid prion disease that is caused by abnormal prion protein (PrPSc). Recent studies have reported that prion family genes showed a strong association with the susceptibility of several types of prion diseases. To date, an association study of the prion‐related protein gene (PRNT) has not been performed in any type of cervid prion disease.
Methods
In the present study, we investigated PRNT polymorphisms in large deer, including 235 elk, 257 red deer and 150 sika deer. We compared genotype, allele and haplotype frequencies of PRNT polymorphisms between CWD‐negative animals and CWD‐positive animals to find an association of PRNT polymorphisms with the susceptibility of CWD.
Results
We found a total of five novel single nucleotide polymorphisms (SNPs) in the cervid PRNT gene. Interestingly, we observed significantly different distributions of genotypes and allele frequencies of three PRNT SNPs, including c.108C>T, c.159+30C>T and c.159+32A>C, between CWD‐negative and CWD‐positive red deer. In addition, significant differences of two haplotype frequencies in red deer were found between the CWD‐negative and CWD‐positive groups. However, the association identified in the red deer was not found in elk and sika deer.
Conclusion
To the best of our knowledge, this report is the first to describe the strong association of PRNT SNPs with the susceptibility of CWD.