Details

Autor(en) / Beteiligte

• Liu, X.‐J.
• Liang, X.‐Y.
• Guo, J.
• Shi, X.‐K.
• Merzendorfer, H.
• Zhu, K. Y.
• Zhang, J.‐Z.

Titel

V‐ATPase subunit a is required for survival and midgut development of Locusta migratoria

Ist Teil von

• Insect molecular biology, 2022-02, Vol.31 (1), p.60-72

Ort / Verlag

Chichester, UK: John Wiley & Sons, Ltd

Erscheinungsjahr

2022

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• The vacuolar‐type H+‐ATPase (V‐ATPase) is an ATP‐dependent proton pump, which regulates various cellular processes. To date, most functional studies on V‐ATPases of insects have focused on subunits of the V1 complex, and there is little information on the VO genes. In this study, two cDNA sequences of LmV‐ATPase a were identified in Locusta migratoria. RT‐qPCR analysis revealed that LmV‐ATPase a1 and LmV‐ATPase a2 are differentially expressed in various tissues and developmental stages. Injection of dsRNA for the common region of LmV‐ATPase a1 and LmV‐ATPase a2 into third‐instar nymphs resulted in a significant suppression of LmV‐ATPase a. The injected nymphs ceased feeding, lost body weight and finally died at a mortality of 98.6%. Furthermore, aberrations of midgut epithelial cells, the accumulation of electron‐lucent vesicles in the cytoplasm, and a partially damaged brush border were observed in dsLmV‐ATPase a‐injected nymphs using transmission electron microscopy. Especially, the mRNA level of wingles, and notch genes were dramatically down‐regulated in the dsLmV‐ATPase a‐injected nymphs. Taken together, our results suggest that LmV‐ATPase a is required for survival and midgut development of L. migratoria. Hence, this gene could be a good target for RNAi‐based control against locusts. Injected dsLmV‐ATPase a into 3rd instar nymphs resulted in a mortality of 98.6%. Nymphs ceased feeding, lost body weight and there was almost no food in midguts. The accumulation of electron‐lucent vesicles in the cytoplasm, and a partially damaged brush border were observed in dsLmV‐ATPase a‐injected nymphs.