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Nuclear receptor HR96 up‐regulates cytochrome P450 for insecticide detoxification in Tribolium castaneum
Pest management science, 2022-01, Vol.78 (1), p.230-239
Kim, In‐Young
Choi, Byungyoon
Park, Woo‐Ram
Kim, Yu‐Ji
Kim, Bo‐Eun
Mun, Seulgi
Choi, Hueng‐Sik
Kim, Don‐Kyu
2022
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Kim, In‐Young
Choi, Byungyoon
Park, Woo‐Ram
Kim, Yu‐Ji
Kim, Bo‐Eun
Mun, Seulgi
Choi, Hueng‐Sik
Kim, Don‐Kyu
Titel
Nuclear receptor HR96 up‐regulates cytochrome P450 for insecticide detoxification in Tribolium castaneum
Ist Teil von
Pest management science, 2022-01, Vol.78 (1), p.230-239
Ort / Verlag
Chichester, UK: John Wiley & Sons, Ltd
Erscheinungsjahr
2022
Quelle
Wiley-Blackwell Journals
Beschreibungen/Notizen
BACKGROUND Red flour beetle, Tribolium castaneum (T. castaneum), is a major agricultural pest that causes significant damage to stored grains and products. Although hormone receptor 96 (HR96) is known to be the single ortholog corresponding to mammalian constitutive androstane receptor and pregnane X receptor, the structural features of Tribolium HR96 (TcHR96) and its role in insecticide‐mediated transcription control of cytochrome P450 enzyme genes in T. castaneum have not been elucidated yet. RESULTS We cloned full‐length complementary DNA encoding TcHR96 and revealed the role of TcHR96 in transcriptional control of cytochrome P450 enzyme genes. Interestingly, genome‐wide transcriptome analysis of HR96‐deficient beetles using RNA sequencing showed a positive correlation between TcHR96 and gene transcription of metabolizing enzymes involved in phase I detoxification processes. Moreover, TcHR96 overexpression significantly increased the promoter activity of genes encoding phase I P450 enzymes such as CYP4Q4, CYP4G7, CYP4BR3, and CYP345A1. Chromatin immunoprecipitation analysis showed that TcHR96 could directly bind to the promoter of gene encoding CYP345A1, an enzyme for metabolizing insecticides in T. castaneum. Furthermore, imidacloprid, a neonicotinoid insecticide, significantly increased gene expression of phase I P450 enzymes in old larvae of T. castaneum, which were reversed by TcHR96 knockdown. Finally, TcHR96 knockdown significantly decreased the resistance of old larvae to imidacloprid concomitant with reduction of imidacloprid‐mediated phase I P450 enzyme gene expression. CONCLUSION TcHR96 plays a major role in transcriptional control of P450 enzyme for imidacloprid detoxification. Controlling TcHR96 might facilitate the regulation of insecticide tolerance in T. castaneum, thus providing a promising new strategy to manage pest beetle populations. © 2021 Society of Chemical Industry. HR96 up‐regulates phase I P450 enzyme gene expression for insecticide detoxification in Tribolium castaneum.
Sprache
Englisch
Identifikatoren
ISSN: 1526-498X
eISSN: 1526-4998
DOI: 10.1002/ps.6626
Titel-ID: cdi_proquest_miscellaneous_2568589227
Format
–
Schlagworte
Animals
,
Beetles
,
Chromatin
,
Complementary DNA
,
Constitutive Androstane Receptor
,
Cytochrome
,
Cytochrome P-450 Enzyme System - genetics
,
Cytochrome P450
,
Cytochromes P450
,
Detoxification
,
Enzymes
,
Gene expression
,
gene regulation
,
Gene sequencing
,
Genes
,
Genomes
,
Grain storage
,
HR96
,
Imidacloprid
,
Immunoprecipitation
,
insecticide
,
Insecticide resistance
,
Insecticide Resistance - genetics
,
Insecticides
,
Insecticides - pharmacology
,
Larvae
,
Pests
,
Receptors
,
Receptors, Cytoplasmic and Nuclear - genetics
,
red flour beetle
,
Stored products
,
Transcription
,
Transcriptomes
,
Tribolium - genetics
,
Tribolium castaneum
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