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Details

Autor(en) / Beteiligte
Titel
Hook‐basal‐body assembly state dictates substrate specificity of the flagellar type‐III secretion system
Ist Teil von
  • Molecular microbiology, 2021-10, Vol.116 (4), p.1189-1200
Ort / Verlag
England: Blackwell Publishing Ltd
Erscheinungsjahr
2021
Quelle
MEDLINE
Beschreibungen/Notizen
  • The assembly of the bacterial flagellum is orchestrated by the secretion of distinct early and late secretion substrates via the flagellar‐specific type‐III secretion system (fT3SS). However, how the fT3SS is able to distinguish between the different (early and late) substrate classes during flagellar assembly remains poorly understood. In this study, we investigated the substrate selectivity and specificity of the fT3SS of Salmonella enterica at different assembly stages. For this, we developed an experimental setup that allowed us to synchronize hook‐basal‐body assembly and to monitor early and late substrate secretion of fT3SSs operating in either early or late secretion mode, respectively. Our results demonstrate that the fT3SS features a remarkable specificity for only the substrates required at the respective assembly stage. No crosstalk of substrates was observed for fT3SSs operating in the opposing secretion mode. We further found that a substantial fraction of fT3SS surprisingly remained in early secretion mode. Our results thus suggest that the secretion substrate specificity switch of the fT3SS is unidirectional and irreversible. The developed secretion substrate reporter system further provides a platform for future investigations of the underlying molecular mechanisms of the elusive substrate recognition of the T3SS. The type‐III secretion system (T3SS) of the bacterial flagellum distinguishes between the different (early and late) substrate proteins throughout the stepwise assembly process. However, the underlying mechanism remains poorly understood. Here, we demonstrate that the flagellar T3SS specifically recognizes and secretes only the substrate proteins required at the respective stage of flagellum assembly, suggesting that the T3SS substrate specificity switch is unidirectional and irreversible.

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