Details

Autor(en) / Beteiligte

• von Essen, Marina Rode
• Ammitzbøll, Cecilie
• Börnsen, Lars
• Sellebjerg, Finn

Titel

Assessment of commonly used methods to determine myelin-reactivity of T cells in multiple sclerosis

Ist Teil von

• Clinical immunology (Orlando, Fla.), 2021-09, Vol.230, p.108817-108817, Article 108817

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2021

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Many studies have analyzed myelin-reactivity of T cells in multiple sclerosis (MS); however, with conflicting results. In this study we compare methods to determine myelin reactivity of T cells and aim to delineate the cause of inconsistency in the literature. Challenging T cells with myelin antigens we found a significant increase in antigen-reactivity of T cells from patients with MS using an ELISpot-assay, in contrast to a CFSE-dilution assay. Comparing the two assays showed that the myelin-reactive T cells detected in the ELISpot-assay originated primarily from effector memory T cells in contrast to the myelin-reactive T cells of the CFSE-assay representing a population of both naïve, central memory and effector memory T cells. This diversity in T cell populations activated in the two assays likely contribute to the discrepancy found in the literature and encourages thorough considerations when choosing an assay to determine antigen-specificity of T cells in future studies. •Increased myelin-reactivity of T cells from patients with RRMS•ELISpot analysis primarily measure myelin-reactivity of effector memory T cells•CFSE-dilution assay measure myelin-reactivity of naïve, central memory and effector memory T cells•The diversity of T cell populations measured likely contribute to the inconsistency in the literature