Details

Autor(en) / Beteiligte

• Li, Qiaoyu
• Wang, Yanlin
• Ling, Ling
• Qiao, Liang
• Chen, Hui
• Ding, Chuanfan
• Yu, Shaoning

Titel

Rapid and specific detection nanoplatform of serum exosomes for prostate cancer diagnosis

Ist Teil von

• Mikrochimica acta (1966), 2021-08, Vol.188 (8), p.283-283, Article 283

Ort / Verlag

Vienna: Springer Vienna

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Tumor exosomes that inherit specific molecules from their parent cells are emerging as ideal biomarkers in cancer diagnostics. Most currently available exosome isolation and detection methods are time-consuming and non-specific; thus, rapid and specific exosome detection methods are needed both clinically and in research. Here, a dual-functional platform is reported composed of reversible conjunction and “off-on” signal responses. Fe 3 O 4 @SiO 2 @TiO 2 particles with high affinity were applied to capture exosomes, and model exosomes could be isolated from solution within 20 min with a capture efficiency of 91.5%. An “on-off” fluorescence response PSMA aptasensor was constructed with improved selectivity to detect tumor exosomes by recording the fluorescence intensity with λ ex/em  = 557/580 nm. The standard curve for detecting tumor exosomes with the aptasensor was calculated as y  = 371.7 x  + 66.17, ranging from 0.05 to 1 × 10 4 particles/μL, with R 2  = 0.9737, and a detection limit of 5 × 10 2 particles/μL in solution. This method was successfully applied to clinical samples, and the results showed better performance in distinguishing prostate cancer patients and healthy samples than the traditional nanoparticle-tracking analysis (NTA) method. This rapid and accurate detection method for prostate cancer may aid in rapid clinical diagnosis. Graphical abstract Integrating quickly TiO 2 -based isolation with sensitive and specific “on-off” detection of PCa exosomes.