Details

Autor(en) / Beteiligte

• Guo, Hui‐juan
• Wang, Li‐jun
• Wang, Chen
• Guo, De‐zheng
• Xu, Bao‐hua
• Guo, Xing‐qi
• Li, Han

Titel

Identification of an Apis cerana zinc finger protein 41 gene and its involvement in the oxidative stress response

Ist Teil von

• Archives of insect biochemistry and physiology, 2021-09, Vol.108 (1), p.e21830-n/a

Ort / Verlag

Hoboken: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Quelle

Wiley Blackwell Single Titles

Beschreibungen/Notizen

• Zinc finger proteins (ZFPs) are a class of transcription factors that contain zinc finger domains and play important roles in growth, aging, and responses to abiotic and biotic stresses. These proteins activate or inhibit gene transcription by binding to single‐stranded DNA or RNA and through RNA/DNA bidirectional binding and protein–protein interactions. However, few studies have focused on the oxidation resistance functions of ZFPs in insects, particularly Apis cerana. In the current study, we identified a ZFP41 gene from A. cerana, AcZFP41, and verified its function in oxidative stress responses. Real‐time quantitative polymerase chain reaction showed that the transcription level of AcZFP41 was upregulated to different degrees during exposure to oxidative stress, including that induced by extreme temperature, UV radiation, or pesticides. In addition, the silencing of AcZFP41 led to changes in the expression patterns of some known antioxidant genes. Moreover, the activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and glutathione S‐transferase (GST) in AcZFP41‐silenced honeybees were higher than those in a control group. In summary, the data indicate that AcZFP41 is involved in the oxidative stress response. The results provide a theoretical basis for further studies of zinc finger proteins and improve our understanding of the antioxidant mechanisms of honeybees. AcZFP41 is involved in oxidative stress. Research Highlights • The expression of AcZFP41 was induced by oxidative stress. • AcZFP41 overexpression enhanced the cell tolerance to oxidative stress. • AcZFP41 knockdown disrupted the original balance and caused oxidative stress.