Details

Autor(en) / Beteiligte

• Zhu, Wei
• Cai, Er‐Li
• Li, Hao‐Zheng
• Wang, Ping
• Shen, Ai‐Guo
• Popp, Jürgen
• Hu, Ji‐Ming

Titel

Precise Encoding of Triple‐Bond Raman Scattering of Single Polymer Nanoparticles for Multiplexed Imaging Application

Ist Teil von

• Angewandte Chemie International Edition, 2021-09, Vol.60 (40), p.21846-21852

Auflage

International ed. in English

Ort / Verlag

Weinheim: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Stimulated Raman scattering (SRS) microscopy in combination with innovative tagging strategies offers great potential as a universal high‐throughput biomedical imaging tool. Here, we report rationally tailored small molecular monomers containing triple‐bond units with large Raman scattering cross‐sections, which can be polymerized at the nanoscale for enhancement of SRS contrast with smaller but brighter optical nanotags with artificial fingerprint output. From this, a class of triple‐bond rich polymer nanoparticles (NPs) was engineered by regulating the relative dosages of three chemically different triple‐bond monomers in co‐polymerization. The bonding strategy allowed for 15 spectrally distinguishable triple‐bond combinations. These accurately structured nano molecular aggregates, rather than long‐chain macromolecules, could establish a universal method for generating small‐sized biological SRS imaging tags with high sensitivity for high‐throughput multi‐color biomedical imaging. Uniform polymer nanoparticles were constructed by green emulsion polymerization, overcoming the disadvantage of large π‐bond conjugation while keeping many triple bonds to realize stimulated Raman scattering imaging. 15 distinguishable triple‐bond combinations were achieved by Raman shift/intensity encoding in the biological silence region (1800–2800 cm−1), thus providing a tool for high‐throughput biomedical imaging.