Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Vaginal bacterial load in the second trimester is associated with early preterm birth recurrence: a nested case–control study
Ist Teil von
BJOG : an international journal of obstetrics and gynaecology, 2021-12, Vol.128 (13), p.2061-2072
Ort / Verlag
England: Wiley Subscription Services, Inc
Erscheinungsjahr
2021
Quelle
MEDLINE
Beschreibungen/Notizen
Objective
To assess the association between vaginal microbiome (VMB) composition and recurrent early spontaneous preterm birth (sPTB)/preterm prelabour rupture of membranes (PPROM).
Design
Nested case–control study.
Setting
UK tertiary referral hospital.
Sample
High‐risk women with previous sPTB/PPROM <34+0 weeks’ gestation who had a recurrence (n = 22) or delivered at ≥37+0 weeks without PPROM (n = 87).
Methods
Vaginal swabs collected between 15 and 22 weeks’ gestation were analysed by 16S rRNA gene sequencing and 16S quantitative PCR.
Main outcome measure
Recurrent early sPTB/PPROM.
Results
Of the 109 high‐risk women, 28 had anaerobic vaginal dysbiosis, with the remainder dominated by lactobacilli (Lactobacillus iners 36/109, Lactobacillus crispatus 23/109, or other 22/109). VMB type and diversity were not associated with recurrence. Women with a recurrence, compared to those without, had a higher median vaginal bacterial load (8.64 versus 7.89 log10 cells/mcl, adjusted odds ratio [aOR] 1.90, 95% CI 1.01–3.56, P = 0.047) and estimated Lactobacillus concentration (8.59 versus 7.48 log10 cells/mcl, aOR 2.35, (95% CI 1.20–4.61, P = 0.013). A higher recurrence risk was associated with higher median bacterial loads for each VMB type after stratification, although statistical significance was reached only for L. iners domination (aOR 3.44, 95% CI 1.06–11.15, P = 0.040). Women with anaerobic dysbiosis or L. iners domination had a higher median vaginal bacterial load than women with a VMB dominated by L. crispatus or other lactobacilli (8.54, 7.96, 7.63, and 7.53 log10 cells/mcl, respectively).
Conclusions
Vaginal bacterial load is associated with early sPTB/PPROM recurrence. Domination by lactobacilli other than L. iners may protect women from developing high bacterial loads. Future PTB studies should quantify vaginal bacteria and yeasts.
Tweetable
Increased vaginal bacterial load in the second trimester may be associated with recurrent early spontaneous preterm birth.
Tweetable
Increased vaginal bacterial load in the second trimester may be associated with recurrent early spontaneous preterm birth.