Details

Autor(en) / Beteiligte

• Fonseca, Paula L.C.
• Mucherino M., Jonathan J.
• Porto, Joel A.M.
• Armache, Juliana N.
• de Almeida, João Paulo P.
• da Silva, Felipe F.
• Olmo, Roenick P.
• Faria, Isaque J. da S.
• de Carvalho, Daniel S.
• Góes-Neto, Aristóteles
• Corrêa, Ronan X.
• Pirovani, Carlos P.
• Pacheco, Luis G.C.
• Costa, Marco Antônio
• Aguiar, Eric R.G.R.

Titel

Genome-wide identification of miRNAs and target regulatory network in the invasive ectoparasitic mite Varroa destructor

Ist Teil von

• Genomics (San Diego, Calif.), 2021-07, Vol.113 (4), p.2290-2303

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Varroa destructor is an ectoparasite mite that attacks bees leading to colony disorders worldwide. microRNAs (miRNAs) are key molecules used by eukaryotes to post-transcriptional control of gene expression. Nevertheless, still lack information aboutV. destructor miRNAs and its regulatory networks. Here, we used an integrative strategy to characterize the miRNAs in the V. destructor mite. We identified 310 precursors that give rise to 500 mature miRNAs, which 257 are likely mite-specific elements. miRNAs showed canonical length ranging between 18 and 25 nucleotides and 5′ uracil preference. Top 10 elements concentrated over 80% of total miRNA expression, with bantam alone representing ~50%. We also detected non-templated bases in precursor-derived small RNAs, indicative of miRNA post-transcriptional regulatory mechanisms. Finally, we note that conserved miRNAs control similar processes in different organisms, suggesting a conservative role. Altogether, our findings contribute to the better understanding of the mite biology that can assist future studies on varroosis control. •Characterization of Varroa destructor miRNAs•Identification of 310 precursors that give rise to 500 mature miRNAs•miRNA can be found in cluster co-regulating many targets.•Existence of miRNA post-transcriptional regulatory mechanisms•Identification of conserved elements that play similar role in different organisms.