Details

Autor(en) / Beteiligte

• Ladd, S. Nemiah
• Nelson, Daniel B.
• Bamberger, Ines
• Daber, L. Erik
• Kreuzwieser, Jürgen
• Kahmen, Ansgar
• Werner, Christiane

Titel

Metabolic exchange between pathways for isoprenoid synthesis and implications for biosynthetic hydrogen isotope fractionation

Ist Teil von

• The New phytologist, 2021-09, Vol.231 (5), p.1708-1719

Ort / Verlag

Lancaster: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Summary Hydrogen isotope ratios of plant lipids are used for paleoclimate reconstruction, but are influenced by both source water and biosynthetic processes. Measuring 2H : 1H ratios of multiple compounds produced by different pathways could allow these effects to be separated, but hydrogen isotope fractionations during isoprenoid biosynthesis remain poorly constrained. To investigate how hydrogen isotope fractionation during isoprenoid biosynthesis is influenced by molecular exchange between the cytosolic and plastidial production pathways, we paired position‐specific 13C‐pyruvate labeling with hydrogen isotope measurements of lipids in Pachira aquatica saplings. We find that acetogenic compounds primarily incorporated carbon from 13C2‐pyruvate, whereas isoprenoids incorporated 13C1‐ and 13C2‐pyruvate equally. This indicates that cytosolic pyruvate is primarily introduced into plastidial isoprenoids via glyceraldehyde 3‐phosphate and that plastidial isoprenoid intermediates are incorporated into cytosolic isoprenoids. Probably as a result of the large differences in hydrogen isotope fractionation between plastidial and cytosolic isoprenoid pathways, sterols from P. aquatica are at least 50‰ less 2H‐enriched relative to phytol than sterols in other plants. These results provide the first experimental evidence that incorporation of plastidial intermediates reduces 2H : 1H ratios of sterols. This suggests that relative offsets between the 2H : 1H ratios of sterols and phytol can trace exchange between the two isoprenoid synthesis pathways.