Details

Autor(en) / Beteiligte

• Joshi, Sumedh
• Fedoseyenko, Dmytro
• Sharma, Vishav
• Nesbit, Mark A
• Britt, R. David
• Begley, Tadhg P

Titel

Menaquinone Biosynthesis: New Strategies to Trap Radical Intermediates in the MqnE-Catalyzed Reaction

Ist Teil von

• Biochemistry (Easton), 2021-06, Vol.60 (21), p.1642-1646

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Aminofutalosine synthase (MqnE) is a radical SAM enzyme that catalyzes the conversion of 3-((1-carboxyvinyl)­oxy)­benzoic acid to aminofutalosine during the futalosine-dependent menaquinone biosynthesis. In this Communication, we report the trapping of a radical intermediate in the MqnE-catalyzed reaction using sodium dithionite, molecular oxygen, or 5,5-dimethyl-1-pyrroline-N-oxide. These radical trapping strategies are potentially of general utility in the study of other radical SAM enzymes.