Details

Autor(en) / Beteiligte

• Molina‐Holgado, Eduardo
• Paniagua‐Torija, Beatriz
• Arevalo‐Martin, Angel
• Moreno‐Luna, Rafael
• Esteban, Pedro F.
• Le, Minh Quynh Uyen
• Del Cerro, Maria del Mar
• Garcia‐Ovejero, Daniel

Titel

Cannabinoid Receptor 1 associates to different molecular complexes during GABAergic neuron maturation

Ist Teil von

• Journal of neurochemistry, 2021-08, Vol.158 (3), p.640-656

Ort / Verlag

England: Blackwell Publishing Ltd

Erscheinungsjahr

2021

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• CB1 cannabinoid receptor is widely expressed in the central nervous system of animals from late prenatal development to adulthood. Appropriate activation and signaling of CB1 cannabinoid receptors in cortical interneurons are crucial during perinatal/postnatal ages and adolescence, when long‐lasting changes in brain activity may elicit subsequent appearance of disorders in the adult brain. Here we used an optimized immunoprecipitation protocol based on specific antibodies followed by shot‐gun proteomics to find CB1 interacting partners in postnatal rat GABAergic cortical neurons in vitro at two different stages of maturation. Besides describing new proteins associated with CB1 like dihydrolipoyllysine‐residue acetyltransferase component of pyruvate dehydrogenase complex (DLAT), fatty acid synthase (FASN), tyrosine 3‐monooxygenase/tryptophan 5‐monooxygenase activation protein zeta (YWHAZ), voltage‐dependent anion channel 1 (VDAC1), myosin phosphatase Rho‐interacting protein (MPRIP) or usher syndrome type‐1C protein‐binding protein 1 (USHBP1), we show that the signaling complex of CB1 is different between maturational stages. Interestingly, the CB1 signaling complex is enriched at the more immature stage in mitochondrial associated proteins and metabolic molecular functions, whereas at more mature stage, CB1 complex is increased in maturation and synaptic‐associated proteins. We describe also interacting partners specifically immunoprecipitated with either N‐terminal or C‐terminal CB1 directed antibodies. Our results highlight new players that may be affected by altered cannabinoid signaling at this critical window of postnatal cortical development. Cannabinoids are lipid mediators that participate in brain development. Appropriate activation and signaling of CB1 cannabinoid receptor are crucial during perinatal/postnatal ages and adolescence when long‐lasting changes in brain activity may elicit subsequent appearance of disorders in the adult brain. Here we describe new molecular partners that associate with CB1 during neuronal postnatal maturation and may mediate the different cannabinoid actions along with the development of brain circuits.