Details

Autor(en) / Beteiligte

• Liu, Zhijia
• Radi, Mohammad
• Mohamed, Elsayed T.T.
• Feist, Adam M.
• Dragone, Giuliano
• Mussatto, Solange I.

Titel

Adaptive laboratory evolution of Rhodosporidium toruloides to inhibitors derived from lignocellulosic biomass and genetic variations behind evolution

Ist Teil von

• Bioresource technology, 2021-08, Vol.333, p.125171-125171, Article 125171

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• •A strategy of adaptive laboratory evolution in hydrolysate-based medium was developed.•Evolved strains of Rhodosporidium toruloides presented better tolerance to inhibitors.•The strain’s ability to accumulate lipids and produce carotenoids was also improved.•Whole genome sequencing revealed tolerance-related genes in the wild-type strain. Using lignocellulosic biomass hydrolysate for the production of microbial lipids and carotenoids is still a challenge due to the poor tolerance of oleaginous yeasts to the inhibitors generated during biomass pretreatment. In this study, a strategy of adaptive laboratory evolution in hydrolysate-based medium was developed to improve the tolerance of Rhodosporidium toruloides to inhibitors present in biomass hydrolysate. The evolved strains presented better performance to grow in hydrolysate medium, with a significant reduction in their lag phases, and improved ability to accumulate lipids and produce carotenoids when compared to the wild-type starting strain. In the best cases, the lag phase was reduced by 72 h and resulted in lipid accumulation of 27.89 ± 0.80% (dry cell weight) and carotenoid production of 14.09 ± 0.12 mg/g (dry cell weight). Whole genome sequencing analysis indicated that the wild-type strain naturally contained tolerance-related genes, which provided a background that allowed the strain to evolve in biomass-derived inhibitors.