Details

Autor(en) / Beteiligte

• Zheng, Cheng
• Qu, Mingjin
• Liu, Yingmiao
• Wang, Junzhi
• Ying, Hanjie

Titel

Design and optimizing a new CDP‐choline in vitro multienzyme producing process starts from d‐ribose

Ist Teil von

• Biotechnology and applied biochemistry, 2022-06, Vol.69 (3), p.1029-1035

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2022

Quelle

Wiley Online Library

Beschreibungen/Notizen

• This work designs an in vitro multienzyme system to produce CDP‐choline from d‐ribose and develop an optimization procedure for one‐pot multienzyme catalytic system. The entire process integrated 10 enzymes, and an efficient acetate kinase/acetyl phosphate‐based ATP regeneration module was applied. Then, some optimizations to this system were made including selecting optimum enzyme building blocks and improving expression parameters. The process improved the final yield of CDP‐choline from 0.2 to 6 g/L (CDP‐choline titer 12.2 mM). This new one‐pot CDP‐choline producing system has a potential for industrial use, and the optimization procedure shed light on improving other one‐pot multienzyme system for industrial production of energy rich compounds.