Details

Autor(en) / Beteiligte

• Yoon, Deok Jun
• Cho, Eui-Sang
• Hwang, Chi Young
• Nam, Young-Do
• Park, So-Lim
• Lim, Seong-Il
• Seo, Myung-Ji

Titel

Nocardioides luti sp. nov., belonging to the family Nocardioidaceae isolated from kaolinite, exhibiting the biosynthesis potential of alkylresorcinol

Ist Teil von

• Antonie van Leeuwenhoek, 2021-07, Vol.114 (7), p.983-995

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A novel Gram-staining-positive, short rod-shaped, non-motile, and non-pigmented actinobacterial strain (KIGAM211 T ) was isolated from kaolinite, a soft white clay mineral, collected from Sancheong in the Republic of Korea. On the basis of 16S rRNA gene sequence analysis, strain KIGAM211 T was determined to belong to the genus Nocardioides and was most closely related to N. ungokensis UKS-03 T (97.5% similarity). Cells could grow between 4 and 35 °C (optimum 30 °C), 0–3% (w/v) NaCl concentration (optimum 0%) and pH 5.5–8.5 (optimum 7.0) on R2A agar. Morphological appearance of colonies was cream-white, arranged singly or in groups. Biochemical characterization of strain KAGAM211 T indicated that it could hydrolyze casein, gelatin, Tweens 40 and tyrosine. Furthermore, the strain was positive for both oxidase and catalase activity. Strain KIGAM211 T was characterized chemotaxonomically by MK-8 (H 4 ) as the predominant menaquinone and phosphatidylglycerol (PG) and phosphatidylinositol (PI) as the major polar lipids. Major fatty acids were iso-C 16:0 and C 18:1 ω9c . The Ortholog average nucleotide identity (OrthoANI) and in silico DNA-DNA hybridization ( is DDH) values between strain KIGAM211T and its most closely related strains of the Nocardioides genus were < 82% and < 24%, respectively, suggesting that strain KIGAM211 T represent a novel species. The whole genome size of KIGAM211 T was 4.52 Mb, comprising a total of 4,294 genes with DNA G + C content of 72.3 mol%. The genome of strain KIGAM211 T also comprises the biosynthetic gene cluster for alkylresorcinol as secondary metabolite. The results of physiological, taxonomical, phylogenetic, and whole genome analyses allowed for differentiation of strain KIGAM211 T from the recognized Nocardioides species. Therefore, strain KIGAM211 T is considered to represent a novel species, for which the name Nocardioides luti sp. nov. (type strain KIGAM211 T  = KCTC 49364 T  = JCM 33859 T ) is proposed.