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Vibrio nitrifigilis sp. nov., a marine nitrogen-fixing bacterium isolated from the lagoon sediment of an islet inside an atoll
Ist Teil von
Antonie van Leeuwenhoek, 2021-07, Vol.114 (7), p.933-945
Ort / Verlag
Cham: Springer International Publishing
Erscheinungsjahr
2021
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
A nitrogen-fixing isolate of facultatively anaerobic, marine bacterium, designated strain NFV-1
T
, was recovered from the lagoon sediment of Dongsha Island, Taiwan. It was a Gram-negative rod which exhibited motility with monotrichous flagellation in broth cultures. The strain required NaCl for growth and grew optimally at about 25–35 °C, 3% NaCl and pH 7–8. It grew aerobically and could achieve anaerobic growth by fermenting
d
-glucose or other carbohydrates as substrates. NH
4
Cl could serve as a sole nitrogen source for growth aerobically and anaerobically, whereas growth with N
2
as the sole nitrogen source was observed only under anaerobic conditions. Cellular fatty acids were predominated by C
16:1
ω
7
c
, C
16:0
, and C
18:1
ω
7
c
. The major polar lipids consisted of phosphatidylethanolamine and phosphatidylserine. Strain NFV-1
T
had a DNA G + C content of 42.5 mol%, as evaluated according to the chromosomal DNA sequencing data. Analyses of sequence similarities and phylogeny based on the 16S rRNA genes, together with the housekeeping genes,
gyrB
,
ftsZ
,
mreB
,
topA
and
gapA
, indicated that the strain formed a distinct species-level lineage in the genus
Vibrio
of the family
Vibrionaceae
. These phylogenetic data and those from genomic and phenotypic characterisations support the establishment of a novel
Vibrio
species, for which the name
Vibrio nitrifigilis
sp. nov. (type strain NFV-1
T
= BCRC 81211
T
= JCM 33628
T
) is proposed.