Details

Autor(en) / Beteiligte

• Hwang, Chi Young
• Cho, Eui-Sang
• Yoon, Deok Jun
• Seo, Myung-Ji

Titel

Halobellus ruber sp. nov., a deep red-pigmented extremely halophilic archaeon isolated from a Korean solar saltern

Ist Teil von

• Antonie van Leeuwenhoek, 2021-07, Vol.114 (7), p.997-1011

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A novel halophilic archaeon, strain MBLA0160 T , was isolated from a solar saltern in Sorae, Republic of Korea. The cells are deep-red pigmented, Gram-negative, rod shaped, motile, and lysed in distilled water. The strain MBLA0160 T grew at 25–45 °C (optimum 37 °C), in 15–30% (w/v) NaCl (optimum 20%) and 0.1–1.0 M MgCl 2 (optimum 0.3–0.5 M) at pH 5.0–9.0 (optimum 7.0). Phylogenetic analysis based on the 16S rRNA sequence showed that this strain was related to two species within the genus Halobellus ( Hbs .), with 98.4% and 95.8% similarity to Hbs. salinus CSW2.24.4  T and Hbs. clavatus TNN18 T , respectively. The major polar lipids of the strain MBLA160 T were phosphatidylglycerol, phosphatidylglycerol sulfate, and phosphatidylglycerol phosphate methyl ester. The genome size, G + C content, and N50 value of MBLA0160 T were 3.49 Mb, 66.5 mol%, and 620,127 bp, respectively. According to predicted functional proteins of strain MBLA0160 T , the highest category was amino acid transport and metabolism. Genome rapid annotation showed that amino acid and derivatives was the most subsystem feature counts. Pan-genomic analysis showed that strain MBLA0160 T had 97 annotated unique KEGG, which were mainly included metabolism and environmental information processing. Ortholog average nucleotide identities (OrthoANI) and in silico DNA-DNA hybridization ( is DDH) values between the strain MBLA0160 T and other strains of the genus Halobellus were under 84,4% and 28.1%, respectively. The genome of strain MBLA0160 T also contain the biosynthetic gene cluster for C 50 carotenoid as secondary metabolite. Based on the phylogenetic, phenotypic, chemotaxonomic properties, and comparative genomic analyses, strain MBLA0160 T is considered to represent a novel species of the genus Halobellus , for which the name Halobellus ruber sp. nov. is proposed. The type strain is MBLA0160 T (= KCTC 4291  T  = JCM 34172  T ).