Details

Autor(en) / Beteiligte

• Cheng, Yue
• Xie, Ying
• Ge, Ji-chun
• Wang, Lei
• Peng, Dai-yin
• Yu, Nian-jun
• Zhang, Yue
• Jiang, Yue-hang
• Luo, Jian-ping
• Chen, Wei-dong

Titel

Structural characterization and hepatoprotective activity of a galactoglucan from Poria cocos

Ist Teil von

• Carbohydrate polymers, 2021-07, Vol.263, p.117979-117979, Article 117979

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •A water-soluble galactoglucan (PCP-1C) was purified from Poria cocos.•The chemical composition and structural features of PCP-1C were characterized.•PCP-1C can effectively inhibit the metabolism of toxicant and reduce liver damage. To find the polysaccharide with hepatoprotective activity from Poria cocos and clarify its structure, a galactoglucan (PCP-1C) with a molecular weight of 17 kDa was purified from the Poria cocos sclerotium by column chromatography and activity evaluation in the present work. It was composed of galactose, glucose, mannose, and fucose in a molar percentage of 43.5: 24.4: 17.4: 14.6. Structural characterization showed that PCP-1C has a backbone consisted of 1,6-α-D-Galp, which branches composed of 1,3-β-D-Glcp, 1,4-β-D-Glcp, 1,6-β-D-Glcp, T-β-D-Glcp, T-α-D-Manp, T-α-L-Fucp and 1,3-α-L-Fucp. In vivo experiments found that PCP-1C can apparently improve the damage of liver tissue in CCl4-treated mice and relieve oxidative stress and inflammation. PCP-1C also reduced the expression of CAR and CYP2E1 in the liver. These findings indicated strong hepatoprotective effect of PCP-1C, which was attributed to the reduction of CCl4 metabolism via inhibiting the CAR/CYP2E1 signal pathway.