Details

Autor(en) / Beteiligte

• Chang, Jaewon
• Baek, Yonugseok
• Lee, Injee
• Sekiguchi, Hiroshi
• Ichiyanagi, Kouhei
• Mio, Kazuhiro
• Nozawa, Shunsuke
• Fukaya, Ryo
• Adachi, Shin-ichi
• Kuramochi, Masahiro
• Sasaki, Yuji C.

Titel

Diffracted X-ray blinking measurements of interleukin 15 receptors in the inner/outer membrane of living NK cells

Ist Teil von

• Biochemical and biophysical research communications, 2021-06, Vol.556, p.53-58

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Interleukin 15 receptor (IL-15R) is a transmembrane signalling protein consisting of 3 subsets: α, β (IL-15Rβ), and γ (γc). IL-2 and IL-15 share the signalling domains IL-15Rβ and γc, although they bind to intrinsic α-subsets and non-signalling domains. Additionally, IL-2 and IL-15 play different roles; therefore, there have been many observations of the dynamic behaviours of IL-15R, which are linked to physiological functions. For more practical discrimination between IL-2 and IL-15, a study was designed and carried out in which α-subsets were removed and a cytoplasmic inhibitor was applied to create a simplified environment in which secondary signalling molecules were reduced. We also applied a new measurement method, diffracted X-ray blinking (DXB), to achieve higher accuracy (<0.01 Å). The dynamics of IL-2 binding (confined motion, max range = 0.71 Å) and IL-15 binding (normal motion) in live natural killer cells were different. We also confirmed. that DXB was a suitable method to quantitatively evaluate the transmembrane protein dynamics of inner/outer live cell membranes by labeling the extracellular domain since the measurements were dependent on the cytosolic environment. •Intramolecular dynamics of IL-2 and IL-15 with sharing receptor were discriminated quantitatively in the live NK cell.•IL-2 binding narrowed the distance between β and γ domain, and changed the diffusion mode in the presence of nifuroxazide.•Diffracted X-ray Blinking (DXB) can determine the functional motion of inner/outer transmembrane proteins.