Details

Autor(en) / Beteiligte

• Katsuya, Hiroo
• Cook, Lucy B M
• Rowan, Aileen G
• Melamed, Anat
• Turpin, Jocelyn
• Ito, Jumpei
• Islam, Saiful
• Miyazato, Paola
• Jek Yang Tan, Benjy
• Matsuo, Misaki
• Miyakawa, Toshikazu
• Nakata, Hirotomo
• Matsushita, Shuzo
• Taylor, Graham P
• Bangham, Charles R M
• Kimura, Shinya
• Satou, Yorifumi

Titel

Clonality of HIV-1– and HTLV-1–Infected Cells in Naturally Coinfected Individuals

Ist Teil von

• The Journal of infectious diseases, 2022-01, Vol.225 (2), p.317-326

Ort / Verlag

US: Oxford University Press

Erscheinungsjahr

2022

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Abstract Background Coinfection with human immunodeficiency virus type 1 (HIV-1) and human T-cell leukemia virus type 1 (HTLV-1) diminishes the value of the CD4+ T-cell count in diagnosing AIDS, and increases the rate of HTLV-1–associated myelopathy. It remains elusive how HIV-1/HTLV-1 coinfection is related to such characteristics. We investigated the mutual effect of HIV-1/HTLV-1 coinfection on their integration sites (ISs) and clonal expansion. Methods We extracted DNA from longitudinal peripheral blood samples from 7 HIV-1/HTLV-1 coinfected, and 12 HIV-1 and 13 HTLV-1 monoinfected individuals. Proviral loads (PVL) were quantified using real-time polymerase chain reaction (PCR). Viral ISs and clonality were quantified by ligation-mediated PCR followed by high-throughput sequencing. Results PVL of both HIV-1 and HTLV-1 in coinfected individuals was significantly higher than that of the respective virus in monoinfected individuals. The degree of oligoclonality of both HIV-1– and HTLV-1–infected cells in coinfected individuals was also greater than in monoinfected subjects. ISs of HIV-1 in cases of coinfection were more frequently located in intergenic regions and transcriptionally silent regions, compared with HIV-1 monoinfected individuals. Conclusions HIV-1/HTLV-1 coinfection makes an impact on the distribution of viral ISs and clonality of virus-infected cells and thus may alter the risks of both HTLV-1– and HIV-1–associated disease. We investigated the mutual effect of HIV-1/HTLV-1 coinfection on their integration sites and the clonal expansion and found the degree of oligoclonality of both HIV-1– and HTLV-1–infected cells in coinfected individuals was greater than that in monoinfected subjects.