Details

Autor(en) / Beteiligte

• Milburn, Jemma V.
• Hoog, Anna M.
• Winkler, Simona
• van Dongen, Katinka A.
• Leitner, Judith
• Patzl, Martina
• Saalmüller, Armin
• de Luca, Karelle
• Steinberger, Peter
• Mair, Kerstin H.
• Gerner, Wilhelm

Titel

Expression of CD9 on porcine lymphocytes and its relation to T cell differentiation and cytokine production

Ist Teil von

• Developmental and comparative immunology, 2021-08, Vol.121, p.104080-104080, Article 104080

Ort / Verlag

United States: Elsevier Ltd

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• In this work, we report on two novel monoclonal antibodies, specific for porcine CD9. CD9 is a tetraspanin that is expressed on a wide variety of cells. We phenotyped porcine immune cell subsets and found that CD9 was expressed on all monocytes as well as a subset of B cells. CD9 was variably expressed on T cells, with CD4 T cells containing the highest frequency of CD9+ cells. CD9 expression positively correlated with the frequency of central memory CD4 T cells in ex vivo PBMC. Therefore, we proceeded to explore CD9 as a marker of T cell function. Here we observed that CD9 was expressed on the vast majority of long-lived influenza A virus-specific effector cells that retained the capacity for cytokine production in response to in vitro recall antigen. Therefore, the new antibodies enable the detection of a cell surface molecule with functional relevance to T cells. Considering the importance of CD9 in membrane remodelling across many cell types, they will also benefit the wider field of swine biomedical research. •Two new monoclonal antibody clones (VIV–3B3 and VIV-2E12) are specific for porcine CD9.•Porcine CD9 is expressed on all monocytes and is variably expressed on lymphocytes.•The frequency of CD9+ CD4 T cells correlates with central memory CD4 T cells.•Long-lived T cells which respond to influenza A virus recall antigen express CD9.