Details

Autor(en) / Beteiligte

• Petruzzi, Massimo
• Lucchese, Alberta
• Contaldo, Maria
• Tampoia, Marilina
• Frassanito, Maria Antonia
• Lauritano, Dorina
• della Vella, Fedora

Titel

ELISA detection of anti‐desmoglein 1 and anti‐desmoglein 3 and indirect immunofluorescence in oral pemphigus: A retrospective study

Ist Teil von

• Oral diseases, 2022-05, Vol.28 (4), p.1149-1156

Ort / Verlag

Denmark: Wiley Subscription Services, Inc

Erscheinungsjahr

2022

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Objective The aim of this study was to test the efficacy of autoantibodies to desmoglein 1 and desmoglein 3 detected by ELISA and indirect immunofluorescence in the diagnosis of oral pemphigus and to correlate the antibody titres with the severity of the disease. Materials and Methods We report a retrospective cohort study of 22 patients with oral pemphigus and 64 controls from a single tertiary centre. Data about histopathological examination, direct immunofluorescence, indirect immunofluorescence and ELISA were analysed. Global validation of ELISA and IIF both alone and combined was established by calculating sensitivity, specificity, accuracy and both positive predictive value and negative predictive value. The relationship between Oral Disease Severity Score values and ELISA titres was analysed using Pearson's coefficient. Results The best diagnostic performance was observed for anti‐desmoglein 3 ELISA. The sensitivity was 75% and specificity 100% and positive predictive value and negative predictive value were 92.5% and accuracy 93.9%. The level of agreement with histopathology + direct immunofluorescence was substantial (k = .758). Anti‐desmoglein 3 titres showed a significant correlation with Oral Disease Severity Score (p < .05). Conclusions Serological tests are commonly employed during clinical practice as adjunctive tools. Anti‐desmoglein 3 ELISA should be considered as a first‐instance diagnostic test for oral pemphigus early detection.