Details

Autor(en) / Beteiligte

• Haer, Manjit
• Strahlendorf, Kirsten
• Payne, Jessie
• Jung, Ryan
• Xiao, Emily
• Mirabel, Clementine
• Rahman, Nausheen
• Kowal, Przemek
• Gemmiti, Gabriel
• Cronin, James T.
• Gable, Tyler
• Park-Lee, Kevin
• Drolet-Vives, Katherine
• Balmer, Matthew
• Kirkitadze, Marina

Titel

PAT solutions to monitor adsorption of Tetanus Toxoid with aluminum adjuvants

Ist Teil von

• Journal of pharmaceutical and biomedical analysis, 2021-05, Vol.198, p.114013-114013, Article 114013

Ort / Verlag

England: Elsevier B.V

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Tetanus Toxoid adsorption reaction monitored by in-line infrared and particle sizing and imaging probes. [Display omitted] •Highlights of PAT Solutions to Monitor Adsorption of Tetanus Toxoid with Aluminum Adjuvants.•In-line measurement of particle size and conformational changes of Tetanus Toxoid upon adsorption onto aluminum adjuvants.•Innovative and non-invasive approach to optimize the adsorption of protein antigens to streamline vaccine formulation.•Has potential to accelerate vaccine development and collect sufficient data for digitization of the formulation process. The focus of this study was to examine the small-scale adsorption process of Tetanus Toxoid (TT) as a model protein antigen to aluminum phosphate (AlPO4) and aluminum oxyhydroxide (AlOOH) adjuvants with real-time monitoring by in-line ReactIR™, ParticleTrack™ based on Focused Beam Reflectance Measurement (FBRM) and EasyViewer™ probes. The adsorption process of AlPO4 and AlOOH with TT using was monitored in the small-scale reactors. Conformational changes in TT were monitored using in-line infrared probe ReactIR, whereas particle formation associated with protein adsorption were measured by particle size, count, and imaging tools, such as ParticleTrack with FBRM and EasyViewer probes. ParticleTrack distribution results and kinetic measurements were also supported by observations made using EasyViewer. In addition to EasyMax, BioBLU reactor was also used for the adsorption experiments. ReactIR with ATR-Fiber probe was effectively able to monitor adsorption progress of TT to AlOOH and to AlPO4. ReactIR, EasyViewer, and ParticleTrack provided detailed mechanistic and kinetic information for reaction of TT with AlPO4 and AlOOH. These in-situ measurements revealed a possible multi-step process for TT to AlPO4 which may be an indication of antigen adsorption.