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Role and mechanism of histone deacetylases in mouse neuronal development
Ist Teil von
Zhongguo dang dai er ke za zhi, 2021-03, Vol.23 (3), p.294-299
Ort / Verlag
China
Erscheinungsjahr
2021
Link zum Volltext
Quelle
Free E-Journal (出版社公開部分のみ）
Beschreibungen/Notizen
To study the role and mechanism of histone deacetylase 1 (HDAC1) and histone deacetylase 2 (HDAC2) in mouse neuronal development.
The mice with Synapsin1-Cre recombinase were bred with
mice to obtain the mice with neuron-specific
conditional knockout (knockout group), and their littermates without
knockout were used as the control group. The general status of the mice was observed and survival curves were plotted. Brain tissue samples were collected from the knockout group and the control group. Western blot and immunohistochemistry were used to measure the protein expression of related neuronal and axonal markers, neuronal nuclear antigen (NeuN), non-phosphorylated neurofilament heavy chain (np-NF200), and phosphorylated neurofilament heavy chain (p-NF200), as well as the downstream effector of the mTOR signaling pathway, phosphorylated S6 ribosomal protein (p-S6).
The mice with
conditional knockout usually died within one month after birth and were significantly smaller than those in the control group, with