Details

Autor(en) / Beteiligte

• Poyraz Tinartas, Esen
• Goncu, Ebru
• Koc, Kamil

Titel

Apoptotic and autophagic characteristics of perivisceral fat body remodeling of the greater wax moth Galleria mellonella and effects of juvenile hormone analog, fenoxycarb, on these processes

Ist Teil von

• Archives of insect biochemistry and physiology, 2021-05, Vol.107 (1), p.e21780-n/a

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• In holometabolous insects, many tissues and organs such as the fat body and midgut undergo a remodeling process during metamorphosis. Larval fat body cells are eliminated by programmed cell death (PCD), while tissue cells that adapt to adult life are formed by stem cells. In this study, we analyzed the features of the remodeling period of Galleria mellonella fat body in terms of PCD types, apoptotic and autophagic cell death characteristics. Besides, the effects of juvenile hormone (JH) on these processes were evaluated under the modified hormonal conditions via applications of JH analog, fenoxycarb. Several hallmarks of apoptotic and autophagic cell death were analyzed by morphological, biochemical, and molecular methods. The results of the present study have ascertained that the degeneration process of larval cells occurs via autophagic cell death accompanied by caspase‐3 activity during the pupal period and it is regulated by 20‐hydroxyecdysone (20HE) mediated by ecdysone receptor B1 (EcR‐B1). Increased activity of the acid phosphatase and upregulation of ATG6 and ATG8 in parallel with the formation of autophagosomes in the fat body of Galleria during the pupal period strongly indicated that autophagy was the key player in the remodeling processes. Programmed cell death characteristics of Galleria mellonella fat body remodeling under the effects of fenoxycarb application. Research Highlights Fenoxycarb application on day 0 of the last larval instar (7. instar) caused extra larval molting and prevented the fat body remodeling process. No acceptable markers of apoptotic cell death properties were detected except for caspase‐3 enzyme activity. The degeneration process of larval cells occurs via autophagic cell death accompanied by caspase‐3 activity during the pupal period. The period is regulated by 20‐hydroxyecdysone (20HE) mediated by ecdysone receptor B1 (EcR‐B1).