Details

Autor(en) / Beteiligte

• Ksiezarek, Magdalena
• Ribeiro, Teresa Gonçalves
• Rocha, Joana
• Grosso, Filipa
• Perovic, Svetlana Ugarcina
• Peixe, Luisa

Titel

Limosilactobacillus urinaemulieris sp. nov. and Limosilactobacillus portuensis sp. nov. isolated from urine of healthy women

Ist Teil von

• International journal of systematic and evolutionary microbiology, 2021-01, Vol.71 (3)

Ort / Verlag

England

Erscheinungsjahr

2021

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Two Gram-stain-positive strains, c9Ua_26_M and c11Ua_112_M , were isolated from voided urine samples from two healthy women. Comparative 16S rRNA gene sequences demonstrated that these novel strains were members of the genus . Phylogenetic analysis based on gene sequences and core genomes showed that each strain formed a separated branch and are closest to DSM 5837 . The average nucleotide identity (ANI) and Genome-to-Genome Distance Calculator (GGDC) values between c9Ua_26_M and the closest relative DSM 5837 were 90.7 and 42.9 %, respectively. The ANI and GGDC values between c11Ua_112_M and the closest relative DSM 5837 were 91.2 and 45.0 %, and those among the strains were 92.9% and 51,0 %, respectively. The major fatty acids were C (40.2 %), C (26.7 %) and C 9 (17.7 %) for strain c9Ua_26_M , and C 9 (38.0 %), C (33.3 %) and C (17.6 %) for strain c11Ua_112_M . The genomic DNA G+C content of strains c9Ua_26_M and c11Ua_112_M was 39.9 and 39.7 mol%, respectively. On the basis of the data presented here, strains c9Ua_26_M and c11Ua_112_M represent two novel species of the genus , for which the names sp. nov. (c9Ua_26_M =CECT 30144 =LMG 31899 ) and sp. nov. (c11Ua_112_M =CECT 30145 =LMG 31898 ) are proposed.