Details

Autor(en) / Beteiligte

• Okita, Yukari
• Zheng, Ling
• Kawanishi, Kunio
• Miyoshi, Hirotoshi
• Yanagihara, Kazuyoshi
• Kato, Mitsuyasu

Titel

Polyvinyl alcohol scaffolds and supplementation support 3D and sphere culturing of human cancer cell lines by reducing apoptosis and promoting cellular proliferation

Ist Teil von

• Genes to cells : devoted to molecular & cellular mechanisms, 2021-05, Vol.26 (5), p.336-343

Ort / Verlag

England: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Three‐dimensional (3D) culturing mimics the heterogeneous cellular conditions of the in vivo tumor microenvironment compared to 2D monolayer‐cultured cells and 3D cultures of established cancer cell lines (sphere culture) or patient‐derived cancer cells (organoid culture) are frequently used for cancer research or drug screening and evaluation. To establish more cost and time‐efficient 3D culture methods for cancer cell lines, we supplemented sphere culture medium with polyvinyl alcohol (PVA) and found that 3D sphere cultures of breast and pancreatic cancer cell lines were significantly increased. Mechanistically, we found that PVA prevented cell death and promoted cellular proliferation while maintaining levels of stemness‐related gene expression. Furthermore, we showed that polyvinyl formal resin (PVF) 3D scaffolds made by cross‐linked PVA can function in serum‐free, long‐term 3D cultures to support maintenance of sphere‐ or tumor‐like cell masses for diverse cancer cell types. Taken together, we demonstrate the effectiveness of PVA and PVF in human cancer cell line culture protocols. In this study, we evaluated the effect of polyvinyl alcohol (PVA) on multiple human cancer cell lines and found that sphere growth was supported by reducing apoptosis and promoting cellular proliferation. We also found that polyvinyl formal resin (PVF) 3D scaffolds made by cross‐linked PVA are useful for long‐term 3D culturing and allow for mass formation even in cell lines that cannot be easily sphere cultured.