Details

Autor(en) / Beteiligte

• Trotta, Adriana
• Cirilli, Margie
• Marinaro, Mariarosaria
• Bosak, Sunčica
• Diakoudi, Georgia
• Ciccarelli, Stefano
• Paci, Serena
• Buonavoglia, Domenico
• Corrente, Marialaura

Titel

Detection of multi-drug resistance and AmpC β-lactamase/extended-spectrum β-lactamase genes in bacterial isolates of loggerhead sea turtles (Caretta caretta) from the Mediterranean Sea

Ist Teil von

• Marine pollution bulletin, 2021-03, Vol.164, p.112015-112015, Article 112015

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Sea turtles are useful sentinels to monitor the dissemination of antimicrobial resistance (AMR) in the marine coastal ecosystems. Forty Gram negative bacteria were isolated from wounds of 52 injured Caretta caretta, living in the Mediterranean Sea. Bacteria were identified using 16S rRNA gene sequencing and tested for susceptibility to 15 antibiotics. In addition, NGS amplicon sequencing was performed to detect the presence of AmpC β-lactamase genes (blaAmpC) and extended-spectrum β-lactamase (ESBL) genes (blaCTX-M,blaSHV,blaTEM). Seventy-five percent of the isolates (30/40 isolates) exhibited multidrug resistance (MDR) phenotypes and 32.5% (13/40 isolates) were confirmed to be positive for at least one gene. The variants of ESBLs genes were blaCTX-M-3,blaTEM-236 and blaSHV-12. Variants of the blaAmpCβ-lactamase gene i.e., blaACT-24, blaACT-2, blaACT-17, blaDHA-4 and blaCMY-37, were also detected. In addition, 4 isolates were found simultaneously harboring CTX and AmpC genes while 2 strains harbored 3 genes (blaACT-2+TEM-236+SHV-12, and blaCTX-M-3+ACT-24+TEM-236). •C. caretta is a sentinel species to monitor the presence of AMR in marine ecosystem.•Forty Gram-negative were tested for antimicrobial susceptibility and ESBL genes.•75% of isolates were MDR and 32.5% were positive for at least one gene.•The detected ESBLs variants were blaCTX-M-3,blaTEM-236 and blaSHV-12.•The strains encoding blaAmpCβ-lactamase gene belonged to ACT, DHA and CMY families.