Details

Autor(en) / Beteiligte

• Mattelaer, Charles‐Alexandre
• Maiti, Mohitosh
• Smets, Laurent
• Maiti, Munmun
• Schepers, Guy
• Mattelaer, Henri‐Philippe
• Rosemeyer, Helmut
• Herdewijn, Piet
• Lescrinier, Eveline

Titel

Stable Hairpin Structures Formed by Xylose‐Based Nucleic Acids

Ist Teil von

• Chembiochem : a European journal of chemical biology, 2021-05, Vol.22 (9), p.1638-1645

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Xenobiology explores synthetic nucleic acid polymers as alternative carriers of genetic information to expand the central dogma. The xylo‐ and deoxyxylo‐nucleic acids (XyNA and dXyNA), containing 3’ epimers of riboses and deoxyriboses, are considered to be potential candidates for an orthogonal system. In this study, thermal and spectroscopic analyses show that XyNA and dXyNA form stable hairpins. The dXyNA hairpin structure determined by NMR spectroscopy contains a flexible loop that locks the stem into a stable ladder‐like duplex with marginal right‐handed helicity. The reduced flexibility of the dXyNA duplex observed in the stem of the hairpin demonstrates that folding of dXyNA yields more stable structures described so far. A stable ladder: Thermal and spectroscopic analyses of xylose (XyNA) and deoxyxylose nucleic acids (dXyNA) indicate the formation of stable hairpin structures. The solution structure of a fully modified dXyNA hairpin indicates the structural preference for a ladder‐like stem with a noncanonical loop.